Hotspot

pafII-cemA

LSC · 60962-61660 · 699 bp

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Pi 0.0215
MarkerSeek score 65.60
Primer available yes
Label pafII-cemA

Feature Metadata

label_name
pafII-cemA
feature_type
igs
region
LSC
length_bp
699
variable_sites
15
pi
0.0215
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
pafII-cemA_p1 TTGGCCTATTTCTTGCGTGT GAGAGAGAAAGCCACCAAGG 45.0 55.0 58.1 57.9 806-826 bp 1.0 79.9
pafII-cemA_p2 ACAAAAAGCTGCGGAATTGG GAGAGAGAAAGCCACCAAGG 45.0 55.0 57.8 57.9 822-842 bp 1.0 79.4
pafII-cemA_p3 GCCATTCCCCTAACTCGTAC GAGAGAGAAAGCCACCAAGG 55.0 55.0 57.8 57.9 872-892 bp 1.0 78.6
pafII-cemA_p4 TGCGGAATTGGCCTATTTCT GAGAGAGAAAGCCACCAAGG 45.0 55.0 57.6 57.9 813-833 bp 1.0 77.1
pafII-cemA_p5 TTGGCCTATTTCTTGCGTGT GCCCAGCATTCCACCAATTA 45.0 50.0 58.1 58.5 856-876 bp 1.0 76.5