Hotspot

accD-psaI

LSC · 59361-59959 · 599 bp

Back to Vinca

Pi 0.0175
MarkerSeek score 58.20
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
599
variable_sites
10
pi
0.0175
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
58.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 GACAGGATTAACCGAGGCTG TTCCAGGAAATTGTGCACCA 55.0 45.0 58.1 57.9 1876-1880 bp 1.0 80.8
accD-psaI_p2 CTTTCGATCGATCCGGGTAC TTCCAGGAAATTGTGCACCA 55.0 45.0 58.0 57.9 1995-1999 bp 1.0 80.7
accD-psaI_p3 CTTTCGATCGATCCGGGTAC ATTTCATCCATCCAGCGTCC 55.0 50.0 58.0 58.0 1592 bp 0.5 59.4
accD-psaI_p4 GACAGGATTAACCGAGGCTG ATTTCATCCATCCAGCGTCC 55.0 50.0 58.1 58.0 1473 bp 0.5 59.3
accD-psaI_p5 TCCCGTAGCAGTTGGAGTTA ATTTCATCCATCCAGCGTCC 50.0 50.0 58.1 58.0 1422 bp 0.5 59.2