Hotspot

trnL-UAA-trnF-GAA

LSC · 47911-48253 · 343 bp

Back to Vinca

Pi 0.0292
MarkerSeek score 68.40
Primer available yes
Label trnL-UAA-trnF-GAA

Feature Metadata

label_name
trnL-UAA-trnF-GAA
feature_type
igs
region
LSC
length_bp
343
variable_sites
10
pi
0.0292
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAA-trnF-GAA_p1 AATCGTGAGGGTTCAAGTCC TCTACCAGCTGAGCTATCCC 50.0 55.0 57.5 58.0 397-408 bp 1.0 78.2
trnL-UAA-trnF-GAA_p2 AATCGTGAGGGTTCAAGTCC TTTGAACTGGTGACACGAGG 50.0 50.0 57.5 58.1 433-444 bp 1.0 77.6
trnL-UAA-trnF-GAA_p3 AATCGTGAGGGTTCAAGTCC GTGCCAGGAACCAGATTTGA 50.0 50.0 57.5 58.1 448-459 bp 1.0 77.2
trnL-UAA-trnF-GAA_p4 AATCGTGAGGGTTCAAGTCC TCAGTCCTCTGCTCTACCAG 50.0 55.0 57.5 57.9 409-420 bp 1.0 77.0
trnL-UAA-trnF-GAA_p5 AATCGTGAGGGTTCAAGTCC CATGTGCCAGGAACCAGATT 50.0 50.0 57.5 58.2 451-462 bp 1.0 76.7