primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	5911	6973	1136-1278	1.000000	80.4
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	0.0	16.9	0.280333	5911	6973	1147-1289	1.000000	79.9
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	20	20	45.0	50.0	58.1	57.3	8.1	16.9	0.806797	5911	6973	1140-1282	1.000000	75.7
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	AAGTCGCACGTTGCTTTCTA	TAAGGCAACGGGTTTTGGTC	20	20	45.0	50.0	58.1	58.7	8.1	0.0	0.818817	5911	6973	1166-1308	1.000000	75.5
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	ATCATGTCCTTCAAGTCGCA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	57.2	58.1	0.0	16.9	0.844354	5911	6973	1148-1290	1.000000	75.3
trnG-UCC_p1	trnG-UCC	1	GGTCCAGTTGTATACAATAATCGC	GATGCACTAAGTCTAGAATATATAAT	24	26	41.7	26.9	57.8	52.5	20.7	0.0	15.757631	8492	9217	802-804	1.000000	41.4
trnG-UCC_p2	trnG-UCC	2	GACAAAAGGTCCAGTTGTATACA	GATGCACTAAGTCTAGAATATATAAT	23	26	39.1	26.9	56.5	52.5	0.1	0.0	16.060322	8492	9217	809-811	1.000000	41.4
trnG-UCC_p3	trnG-UCC	3	ACAAAAGGTCCAGTTGTATACA	GATGCACTAAGTCTAGAATATATAAT	22	26	36.4	26.9	55.3	52.5	0.1	0.0	16.236174	8492	9217	808-810	1.000000	41.4
trnG-UCC_p4	trnG-UCC	4	GTCCAGTTGTATACAATAATCGCA	GATGCACTAAGTCTAGAATATATAAT	24	26	37.5	26.9	57.2	52.5	20.7	0.0	16.371409	8492	9217	801-803	1.000000	41.4
trnG-UCC_p5	trnG-UCC	5	GACAAAAGGTCCAGTTGTATACAA	GATGCACTAAGTCTAGAATATATAAT	24	26	37.5	26.9	57.1	52.5	14.3	0.0	16.404182	8492	9217	809-811	1.000000	41.4
psbM-trnD-GUC_p1	psbM-trnD-GUC	1	AGGCGGTAGGAACTAGAATGA	TTCAATTGGTCAGAGCACCG	21	20	47.6	50.0	58.0	58.5	0.0	5.4	1.505426	29546	30402	982-991	1.000000	69.9
psbM-trnD-GUC_p2	psbM-trnD-GUC	2	AGAAAGGCGGTAGGAACTAGA	TTCAATTGGTCAGAGCACCG	21	20	47.6	50.0	57.9	58.5	0.0	5.4	1.574673	29546	30402	986-995	1.000000	69.3
psbM-trnD-GUC_p3	psbM-trnD-GUC	3	AGTAGAAAGGCGGTAGGAACT	TTCAATTGGTCAGAGCACCG	21	20	47.6	50.0	58.2	58.5	0.0	5.4	1.659667	29546	30402	989-998	1.000000	68.7
psbM-trnD-GUC_p4	psbM-trnD-GUC	4	AGTAGAAAGGCGGTAGGAAC	TTCAATTGGTCAGAGCACCG	20	20	50.0	50.0	56.7	58.5	0.0	5.4	1.821422	29546	30402	989-998	1.000000	67.4
psbM-trnD-GUC_p5	psbM-trnD-GUC	5	GTAGAAAGGCGGTAGGAACT	TTCAATTGGTCAGAGCACCG	20	20	50.0	50.0	56.7	58.5	0.0	5.4	1.821422	29546	30402	988-997	1.000000	67.4
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	20	20	55.0	55.0	57.9	58.2	0.0	10.2	0.381189	46540	47324	846-850	1.000000	78.1
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	TAGGAAAAAGCCGGCTATCG	CCGTAGCGTCTACCGATTTC	20	20	50.0	55.0	57.8	58.2	33.1	10.2	0.482748	46540	47324	897-901	1.000000	77.2
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	20	20	50.0	55.0	57.8	58.2	15.5	10.2	0.488942	46540	47324	878-882	1.000000	77.2
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GCGATGCTCTAACCTCTGAG	CGTCTACCGATTTCGCCATA	20	20	55.0	50.0	57.9	57.6	0.0	0.0	0.543526	46540	47324	840-844	1.000000	76.8
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GCGATGCTCTAACCTCTGAG	GGTTTCCATACCAAGGCTCA	20	20	55.0	50.0	57.9	57.5	0.0	0.0	0.640147	46540	47324	878-882	1.000000	76.0
trnL-UAA-trnF-GAA_p1	trnL-UAA-trnF-GAA	1	AATCGTGAGGGTTCAAGTCC	TCTACCAGCTGAGCTATCCC	20	20	50.0	55.0	57.5	58.0	0.0	17.4	0.476915	47911	48253	397-408	1.000000	78.2
trnL-UAA-trnF-GAA_p2	trnL-UAA-trnF-GAA	2	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	20	20	50.0	50.0	57.5	58.1	0.0	20.1	0.526305	47911	48253	433-444	1.000000	77.6
trnL-UAA-trnF-GAA_p3	trnL-UAA-trnF-GAA	3	AATCGTGAGGGTTCAAGTCC	GTGCCAGGAACCAGATTTGA	20	20	50.0	50.0	57.5	58.1	0.0	0.0	0.564710	47911	48253	448-459	1.000000	77.2
trnL-UAA-trnF-GAA_p4	trnL-UAA-trnF-GAA	4	AATCGTGAGGGTTCAAGTCC	TCAGTCCTCTGCTCTACCAG	20	20	50.0	55.0	57.5	57.9	0.0	0.0	0.610708	47911	48253	409-420	1.000000	77.0
trnL-UAA-trnF-GAA_p5	trnL-UAA-trnF-GAA	5	AATCGTGAGGGTTCAAGTCC	CATGTGCCAGGAACCAGATT	20	20	50.0	50.0	57.5	58.2	0.0	0.0	0.631042	47911	48253	451-462	1.000000	76.7
accD-psaI_p1	accD-psaI	1	GACAGGATTAACCGAGGCTG	TTCCAGGAAATTGTGCACCA	20	20	55.0	45.0	58.1	57.9	0.0	15.7	0.137845	59361	59959	1876-1880	1.000000	80.8
accD-psaI_p2	accD-psaI	2	CTTTCGATCGATCCGGGTAC	TTCCAGGAAATTGTGCACCA	20	20	55.0	45.0	58.0	57.9	20.2	15.7	0.126849	59361	59959	1995-1999	1.000000	80.7
accD-psaI_p3	accD-psaI	3	CTTTCGATCGATCCGGGTAC	ATTTCATCCATCCAGCGTCC	20	20	55.0	50.0	58.0	58.0	20.2	0.0	0.074807	59361	59959	1592-1592	0.500000	59.4
accD-psaI_p4	accD-psaI	4	GACAGGATTAACCGAGGCTG	ATTTCATCCATCCAGCGTCC	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.085803	59361	59959	1473-1473	0.500000	59.3
accD-psaI_p5	accD-psaI	5	TCCCGTAGCAGTTGGAGTTA	ATTTCATCCATCCAGCGTCC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.099641	59361	59959	1422-1422	0.500000	59.2
pafII-cemA_p1	pafII-cemA	1	TTGGCCTATTTCTTGCGTGT	GAGAGAGAAAGCCACCAAGG	20	20	45.0	55.0	58.1	57.9	3.3	0.0	0.202190	60962	61660	806-826	1.000000	79.9
pafII-cemA_p2	pafII-cemA	2	ACAAAAAGCTGCGGAATTGG	GAGAGAGAAAGCCACCAAGG	20	20	45.0	55.0	57.8	57.9	0.0	0.0	0.259844	60962	61660	822-842	1.000000	79.4
pafII-cemA_p3	pafII-cemA	3	GCCATTCCCCTAACTCGTAC	GAGAGAGAAAGCCACCAAGG	20	20	55.0	55.0	57.8	57.9	0.0	0.0	0.342322	60962	61660	872-892	1.000000	78.6
pafII-cemA_p4	pafII-cemA	4	TGCGGAATTGGCCTATTTCT	GAGAGAGAAAGCCACCAAGG	20	20	45.0	55.0	57.6	57.9	13.8	0.0	0.541849	60962	61660	813-833	1.000000	77.1
pafII-cemA_p5	pafII-cemA	5	TTGGCCTATTTCTTGCGTGT	GCCCAGCATTCCACCAATTA	20	20	45.0	50.0	58.1	58.5	3.3	0.0	0.611675	60962	61660	856-876	1.000000	76.5
petA-psbJ_p1	petA-psbJ	1	GAGAAGGTTCAATTGGCCGA	ATTCCGGATTGGGTTCATCC	20	20	50.0	50.0	58.2	57.6	5.4	4.1	0.543756	63544	64621	1134-1137	1.000000	77.4
petA-psbJ_p2	petA-psbJ	2	TCGAGAAGGTTCAATTGGCC	ATTCCGGATTGGGTTCATCC	20	20	50.0	50.0	58.2	57.6	5.4	4.1	0.543756	63544	64621	1136-1139	1.000000	77.4
petA-psbJ_p3	petA-psbJ	3	AGAAGGTTCAATTGGCCGAA	ATTCCGGATTGGGTTCATCC	20	20	45.0	50.0	57.7	57.6	5.4	4.1	0.651337	63544	64621	1133-1136	1.000000	76.6
petA-psbJ_p4	petA-psbJ	4	GGTTCAATTGGCCGAAATGA	ATTCCGGATTGGGTTCATCC	20	20	45.0	50.0	57.0	57.6	5.4	4.1	1.393062	63544	64621	1129-1132	1.000000	70.6
petA-psbJ_p5	petA-psbJ	5	GAGAAGGTTCAATTGGCCGA	TGGTTCATATTCCGGATTGGG	20	21	50.0	47.6	58.2	57.8	5.4	4.1	1.400956	63544	64621	1142-1145	1.000000	70.5
psbF-psbE_p1	psbF-psbE	1	GAACAGCCAACCATCGTACT	AACTGGCCGTTTTGATCCTT	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.099386	65148	65156	116-116	1.000000	79.9
psbF-psbE_p2	psbF-psbE	2	GAACAGCCAACCATCGTACT	ACTGGCCGTTTTGATCCTTT	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.099386	65148	65156	115-115	1.000000	79.9
psbF-psbE_p3	psbF-psbE	3	GAACAGCCAACCATCGTACT	CCGGCAGGGAATTCCATTAA	20	20	50.0	50.0	57.9	57.9	0.0	25.7	0.160661	65148	65156	137-137	1.000000	79.3
psbF-psbE_p4	psbF-psbE	4	AACAGCCAACCATCGTACTG	AACTGGCCGTTTTGATCCTT	20	20	50.0	45.0	58.2	58.0	0.0	0.0	0.196213	65148	65156	115-115	1.000000	79.1
psbF-psbE_p5	psbF-psbE	5	AACAGCCAACCATCGTACTG	ACTGGCCGTTTTGATCCTTT	20	20	50.0	45.0	58.2	58.0	0.0	0.0	0.196213	65148	65156	114-114	1.000000	79.1
ycf15-trnL-CAA_p1	ycf15-trnL-CAA	1	TGAACAGCGAACAACCAGTT	AAATGGTAGACACGCGAGAC	20	20	45.0	50.0	58.3	58.0	0.0	15.9	0.262598	94202	94602	538-538	1.000000	78.8
ycf15-trnL-CAA_p2	ycf15-trnL-CAA	2	TGAACAGCGAACAACCAGTT	TGAAATGGTAGACACGCGAG	20	20	45.0	50.0	58.3	58.0	0.0	15.9	0.263969	94202	94602	540-540	1.000000	78.8
ycf15-trnL-CAA_p3	ycf15-trnL-CAA	3	TGAACAGCGAACAACCAGTT	GCCTTGGTGGTGAAATGGTA	20	20	45.0	50.0	58.3	58.1	0.0	0.0	0.336896	94202	94602	550-550	1.000000	78.2
ycf15-trnL-CAA_p4	ycf15-trnL-CAA	4	TGAACAGCGAACAACCAGTT	TGGTGAAATGGTAGACACGC	20	20	45.0	50.0	58.3	58.2	0.0	0.0	0.451434	94202	94602	543-543	1.000000	77.3
ycf15-trnL-CAA_p5	ycf15-trnL-CAA	5	TGAACAGCGAACAACCAGTT	GAAATGGTAGACACGCGAGA	20	20	45.0	50.0	58.3	57.7	0.0	15.9	0.528137	94202	94602	539-539	1.000000	76.7
ycf1_p1	ycf1	1	GAGCTACTGAGGAACAACGG	TCCTTTCAATCAAGCGGGAG	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.116191	109347	110384	1940-1971	1.000000	80.5
ycf1_p2	ycf1	2	CAATTGTGACCTCTCGGGAG	TCCTTTCAATCAAGCGGGAG	20	20	55.0	50.0	58.0	57.9	4.9	0.0	0.129596	109347	110384	2276-2307	1.000000	80.3
ycf1_p3	ycf1	3	GCGAGCCCCTTTATTCTGAA	TCCTTTCAATCAAGCGGGAG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.152247	109347	110384	2050-2081	1.000000	80.2
ycf1_p4	ycf1	4	AAGCGAGCCCCTTTATTCTG	TCCTTTCAATCAAGCGGGAG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.157983	109347	110384	2052-2083	1.000000	80.1
ycf1_p5	ycf1	5	TTGTGACCTCTCGGGAGAAT	TCCTTTCAATCAAGCGGGAG	20	20	50.0	50.0	58.1	57.9	15.7	0.0	0.175276	109347	110384	2273-2304	1.000000	79.9
ycf1-ndhF_p1	ycf1-ndhF	1	CAACTTCCGAGATGAAGGGG	TCCTTTCAATCAAGCGGGAG	20	20	55.0	50.0	58.0	57.9	5.8	0.0	0.143699	110385	110400	787-818	1.000000	82.1
ycf1-ndhF_p2	ycf1-ndhF	2	AACTTCCGAGATGAAGGGGA	TCCTTTCAATCAAGCGGGAG	20	20	50.0	50.0	58.0	57.9	5.8	0.0	0.161239	110385	110400	786-817	1.000000	82.0
ycf1-ndhF_p3	ycf1-ndhF	3	CAACTTCCGAGATGAAGGGG	CCTTTCAATCAAGCGGGAGT	20	20	55.0	50.0	58.0	58.2	5.8	0.0	0.203453	110385	110400	786-817	1.000000	81.7
ycf1-ndhF_p4	ycf1-ndhF	4	AACTTCCGAGATGAAGGGGA	CCTTTCAATCAAGCGGGAGT	20	20	50.0	50.0	58.0	58.2	5.8	0.0	0.220994	110385	110400	785-816	1.000000	81.5
ycf1-ndhF_p5	ycf1-ndhF	5	AGGGTGGAAAGTGAGGAAGA	TCCTTTCAATCAAGCGGGAG	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.232264	110385	110400	827-858	1.000000	81.3
ccsA-ndhD_p1	ccsA-ndhD	1	AGTTATGGTTCGTTTATATTAACATCT	ACCCGGATTTCGTTCTTTCA	27	20	25.9	45.0	55.3	57.4	5.5	0.7	10.240219	115093	115334	346-346	1.000000	43.7
ccsA-ndhD_p2	ccsA-ndhD	2	AGTTATGGTTCGTTTATATTAACA	ACCCGGATTTCGTTCTTTCA	24	20	25.0	45.0	52.3	57.4	5.5	0.7	10.257075	115093	115334	346-346	1.000000	43.7
ccsA-ndhD_p3	ccsA-ndhD	3	ACATAGTTATGGTTCGTTTATATT	ACCCGGATTTCGTTCTTTCA	24	20	25.0	45.0	52.1	57.4	4.3	0.7	10.466082	115093	115334	350-350	1.000000	43.7
ccsA-ndhD_p4	ccsA-ndhD	4	AGTTATGGTTCGTTTATATTAACATC	ACCCGGATTTCGTTCTTTCA	26	20	26.9	45.0	54.1	57.4	5.5	0.7	10.492324	115093	115334	346-346	1.000000	43.7
ccsA-ndhD_p5	ccsA-ndhD	5	GTTATGGTTCGTTTATATTAACATCT	ACCCGGATTTCGTTCTTTCA	26	20	26.9	45.0	54.1	57.4	5.5	0.7	10.492324	115093	115334	345-345	1.000000	43.7
ndhD_p1	ndhD	1	CCATCCGGGGTTCAAATTCA	GATACCCTGGGACGGATGTA	20	20	50.0	55.0	58.2	58.0	0.0	22.2	0.159364	115335	116486	1915-1916	1.000000	79.6
ndhD_p2	ndhD	2	CCATCCGGGGTTCAAATTCA	AGCTACTTTATCGGCTTGGC	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.199066	115335	116486	1337-1337	1.000000	79.4
ndhD_p3	ndhD	3	GGTGCAAATTCGGCAATTGT	GATACCCTGGGACGGATGTA	20	20	45.0	55.0	58.2	58.0	7.4	22.2	0.210858	115335	116486	2150-2151	1.000000	79.4
ndhD_p4	ndhD	4	TGCAAATTCGGCAATTGTGG	GATACCCTGGGACGGATGTA	20	20	45.0	55.0	58.2	58.0	7.4	22.2	0.212112	115335	116486	2148-2149	1.000000	79.3
ndhD_p5	ndhD	5	CCATCCGGGGTTCAAATTCA	TTCGGGTTTATTTAGGGGCG	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.195245	115335	116486	1805-1805	0.500000	58.4
ndhE-ndhG_p1	ndhE-ndhG	1	GACACATAAGGGCTCTAACCA	CAATTGCTGTTGCTCGTCAA	21	20	47.6	45.0	57.1	57.6	0.0	0.0	2.310985	117754	117863	233-234	1.000000	62.2
ndhE-ndhG_p2	ndhE-ndhG	2	ACAAGTACATGTTCGAGCATCA	CAATTGCTGTTGCTCGTCAA	22	20	40.9	45.0	58.1	57.6	12.2	0.0	2.478886	117754	117863	156-157	1.000000	61.2
ndhE-ndhG_p3	ndhE-ndhG	3	AGTTCAAGACACATAAGGGCTC	CAATTGCTGTTGCTCGTCAA	22	20	45.5	45.0	58.1	57.6	0.0	0.0	2.460178	117754	117863	240-241	1.000000	61.0
ndhE-ndhG_p4	ndhE-ndhG	4	GTTCAAGACACATAAGGGCTCT	CAATTGCTGTTGCTCGTCAA	22	20	45.5	45.0	58.1	57.6	0.0	0.0	2.460178	117754	117863	239-240	1.000000	61.0
ndhE-ndhG_p5	ndhE-ndhG	5	AGTTCAAGACACATAAGGGCT	CAATTGCTGTTGCTCGTCAA	21	20	42.9	45.0	56.9	57.6	0.0	0.0	2.482321	117754	117863	240-241	1.000000	60.8
trnL-CAA-ycf15_p1	trnL-CAA-ycf15	1	AAATGGTAGACACGCGAGAC	TGAACAGCGAACAACCAGTT	20	20	50.0	45.0	58.0	58.3	15.9	0.0	0.262598	143675	144075	538-538	1.000000	78.8
trnL-CAA-ycf15_p2	trnL-CAA-ycf15	2	TGAAATGGTAGACACGCGAG	TGAACAGCGAACAACCAGTT	20	20	50.0	45.0	58.0	58.3	15.9	0.0	0.263969	143675	144075	540-540	1.000000	78.8
trnL-CAA-ycf15_p3	trnL-CAA-ycf15	3	GCCTTGGTGGTGAAATGGTA	TGAACAGCGAACAACCAGTT	20	20	50.0	45.0	58.1	58.3	0.0	0.0	0.336896	143675	144075	550-550	1.000000	78.2
trnL-CAA-ycf15_p4	trnL-CAA-ycf15	4	TGGTGAAATGGTAGACACGC	TGAACAGCGAACAACCAGTT	20	20	50.0	45.0	58.2	58.3	0.0	0.0	0.451434	143675	144075	543-543	1.000000	77.3
trnL-CAA-ycf15_p5	trnL-CAA-ycf15	5	GAAATGGTAGACACGCGAGA	TGAACAGCGAACAACCAGTT	20	20	50.0	45.0	57.7	58.3	15.9	0.0	0.528137	143675	144075	539-539	1.000000	76.7