Hotspot

rpoA

LSC · 78734-79735 · 1002 bp

Pi 0.016

MarkerSeek score 55.00

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: LSC
length_bp: 1002
variable_sites: 24
pi: 0.016
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	CAAAATCCATTTCGCCGTCC	TCATGATAAAAGGGCCTGGC	50.0	50.0	58.1	57.9	1570 bp	0.666667	65.9
rpoA_p2	CCAAAATCCATTTCGCCGTC	TCATGATAAAAGGGCCTGGC	50.0	50.0	58.1	57.9	1571 bp	0.666667	65.9
rpoA_p3	CAAAATCCATTTCGCCGTCC	GTACGAGGTCGGGTGATTTC	50.0	55.0	58.1	58.1	1712 bp	0.666667	65.7
rpoA_p4	CCAAAATCCATTTCGCCGTC	GTACGAGGTCGGGTGATTTC	50.0	55.0	58.1	58.1	1713 bp	0.666667	65.7
rpoA_p5	CAAAATCCATTTCGCCGTCC	CTTGCGGATTCAAGGGTACA	50.0	50.0	58.1	57.9	1675 bp	0.666667	65.5