Hotspot

clpP

LSC · 70715-72774 · 2060 bp

Back to Vigna

Pi 0.0139
MarkerSeek score 53.00
Primer available yes
Label clpP

Feature Metadata

label_name
clpP
feature_type
gene
region
LSC
length_bp
2060
variable_sites
42
pi
0.0139
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
clpP_p1 CTGAACGAGTCGCACATACA AACAGGGTCAGAGGGATCAA 50.0 50.0 58.0 58.0 2963-2971 bp 1.0 81.6
clpP_p2 CTGAACGAGTCGCACATACA ACAGGGTCAGAGGGATCAAA 50.0 50.0 58.0 58.0 2962-2970 bp 1.0 81.6
clpP_p3 CTGAACGAGTCGCACATACA TCACCCCAATGACCCATTTG 50.0 50.0 58.0 58.1 2580-2581 bp 0.666667 66.6
clpP_p4 AGAATAACCTTTGCCGCCAA AACAGGGTCAGAGGGATCAA 45.0 50.0 58.1 58.0 2643-2644 bp 0.666667 66.2
clpP_p5 AGAATAACCTTTGCCGCCAA ACAGGGTCAGAGGGATCAAA 45.0 50.0 58.1 58.0 2642-2643 bp 0.666667 66.2