Hotspot

rps12-rps7

IRa · 137367-137420 · 54 bp

Back to Vigna

Pi 0.0247
MarkerSeek score 67.60
Primer available yes
Label rps12-rps7

Feature Metadata

label_name
rps12-rps7
feature_type
igs
region
IRa
length_bp
54
variable_sites
2
pi
0.0247
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps12-rps7_p1 CCACCCTACAATATGGTGCC TTTCTTCTGCAGTACCTCGC 55.0 50.0 58.0 57.9 117 bp 1.0 80.6
rps12-rps7_p2 CTGTGGAAAGCCGTATTCGA TTTCTTCTGCAGTACCTCGC 50.0 50.0 58.0 57.9 187 bp 1.0 80.2
rps12-rps7_p3 TCTGTGGAAAGCCGTATTCG TTTCTTCTGCAGTACCTCGC 50.0 50.0 58.0 57.9 188 bp 1.0 80.2
rps12-rps7_p4 CTGTGGAAAGCCGTATTCGA ATTGGATCGGATTTTGCGGT 50.0 45.0 58.0 58.2 209 bp 1.0 78.8
rps12-rps7_p5 TCTGTGGAAAGCCGTATTCG ATTGGATCGGATTTTGCGGT 50.0 45.0 58.0 58.2 210 bp 1.0 78.8