Hotspot

ndhE-ndhG

SSC · 114877-115094 · 218 bp

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Pi 0.0214
MarkerSeek score 70.90
Primer available yes
Label ndhE-ndhG

Feature Metadata

label_name
ndhE-ndhG
feature_type
igs
region
SSC
length_bp
218
variable_sites
7
pi
0.0214
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
70.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhE-ndhG_p1 CAAAACAAGTGCATGCTCGA AGGTGCAATTTTTGTGGCTC 45.0 45.0 57.6 57.5 264-273 bp 1.0 77.3
ndhE-ndhG_p2 TCAAAACAAGTGCATGCTCG AGGTGCAATTTTTGTGGCTC 45.0 45.0 57.6 57.5 265-274 bp 1.0 77.2
ndhE-ndhG_p3 TTTCGGCTCGTGATCAATCC AGGTGCAATTTTTGTGGCTC 50.0 45.0 58.3 57.5 314-323 bp 1.0 77.0
ndhE-ndhG_p4 CGGCTCGTGATCAATCCATA AGGTGCAATTTTTGTGGCTC 50.0 45.0 57.6 57.5 311-320 bp 1.0 76.3
ndhE-ndhG_p5 TTTCGGCTCGTGATCAATCC GGTGCAATTTTTGTGGCTCG 50.0 50.0 58.3 58.9 313-322 bp 1.0 74.3