Hotspot

ndhD-psaC

SSC · 113896-114013 · 118 bp

Back to Vigna

Pi 0.0508
MarkerSeek score 71.60
Primer available yes
Label ndhD-psaC

Feature Metadata

label_name
ndhD-psaC
feature_type
igs
region
SSC
length_bp
118
variable_sites
9
pi
0.0508
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
71.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhD-psaC_p1 TGTTAACCAAGGAAAATAATTCGT AAACAACTCGAAGCATGGGT 29.2 45.0 55.3 58.0 174 bp 1.0 44.1
ndhD-psaC_p2 TGTTGTTAACCAAGGAAAATAATTCG AAACAACTCGAAGCATGGGT 30.8 45.0 57.2 58.0 177 bp 1.0 44.1
ndhD-psaC_p3 GTTGTTAACCAAGGAAAATAATTCGT AAACAACTCGAAGCATGGGT 30.8 45.0 57.2 58.0 176 bp 1.0 44.1
ndhD-psaC_p4 TGTTAACCAAGGAAAATAATTCGT GGCATGAAACAACTCGAAGC 29.2 50.0 55.3 58.0 180 bp 1.0 44.0
ndhD-psaC_p5 TGTTGTTAACCAAGGAAAATAATTCG GGCATGAAACAACTCGAAGC 30.8 50.0 57.2 58.0 183 bp 1.0 43.9