Hotspot

trnG-UCC-trnS-GCU

LSC · 55525-56088 · 564 bp

Back to Vigna

Pi 0.0226
MarkerSeek score 63.50
Primer available yes
Label trnG-UCC-trnS-GCU

Feature Metadata

label_name
trnG-UCC-trnS-GCU
feature_type
igs
region
LSC
length_bp
564
variable_sites
19
pi
0.0226
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnS-GCU_p1 TCCTTGAAACGAATCGCACT ACGGAAAGAGAGGGATTCGA 45.0 50.0 57.8 58.2 688-694 bp 1.0 81.3
trnG-UCC-trnS-GCU_p2 GTCCTTGAAACGAATCGCAC ACGGAAAGAGAGGGATTCGA 50.0 50.0 57.8 58.2 689-695 bp 1.0 80.8
trnG-UCC-trnS-GCU_p3 TCCTTGAAACGAATCGCACT ATCCGACGCTATAGTCCACT 45.0 50.0 57.8 57.7 633-639 bp 1.0 80.7
trnG-UCC-trnS-GCU_p4 TCCTTGAAACGAATCGCACT GCAATCCGACGCTATAGTCC 45.0 55.0 57.8 58.3 636-642 bp 1.0 80.4
trnG-UCC-trnS-GCU_p5 GTCCTTGAAACGAATCGCAC ATCCGACGCTATAGTCCACT 50.0 50.0 57.8 57.7 634-640 bp 1.0 80.1