Hotspot

trnE-UUC-trnY-GUA

LSC · 33355-33422 · 68 bp

Back to Vigna

Pi 0.0392
MarkerSeek score 68.70
Primer available yes
Label trnE-UUC-trnY-GUA

Feature Metadata

label_name
trnE-UUC-trnY-GUA
feature_type
igs
region
LSC
length_bp
68
variable_sites
4
pi
0.0392
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnY-GUA_p1 AAGTATGCCCCCATCGTCTA CCAGCGTAGACATATTGCCA 50.0 50.0 57.9 57.8 211 bp 1.0 78.8
trnE-UUC-trnY-GUA_p2 AAGTATGCCCCCATCGTCTA ACCAGCGTAGACATATTGCC 50.0 50.0 57.9 57.8 212 bp 1.0 78.8
trnE-UUC-trnY-GUA_p3 AAGTATGCCCCCATCGTCTA CGAATTTACAGTCCGTCCCC 50.0 55.0 57.9 58.4 190 bp 1.0 78.1
trnE-UUC-trnY-GUA_p4 AAGTATGCCCCCATCGTCTA ACGAATTTACAGTCCGTCCC 50.0 50.0 57.9 57.6 191 bp 1.0 77.9
trnE-UUC-trnY-GUA_p5 CTCTCTTTCAAGGAGGCAGC CCAGCGTAGACATATTGCCA 55.0 50.0 58.3 57.8 177 bp 1.0 77.8