Hotspot

trnT-GGU-trnE-UUC

LSC · 32498-33281 · 784 bp

Back to Vigna

Pi 0.02
MarkerSeek score 66.90
Primer available yes
Label trnT-GGU-trnE-UUC

Feature Metadata

label_name
trnT-GGU-trnE-UUC
feature_type
igs
region
LSC
length_bp
784
variable_sites
23
pi
0.02
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-trnE-UUC_p1 GAACCGATGACTTACGCCTT GCTGCCTCCTTGAAAGAGAG 50.0 55.0 58.0 58.3 856-887 bp 1.0 83.0
trnT-GGU-trnE-UUC_p2 GAACCGATGACTTACGCCTT TGTCCTGAACCACTAGACGA 50.0 50.0 58.0 57.7 835-866 bp 1.0 82.9
trnT-GGU-trnE-UUC_p3 CCATGGCGTTACTCTACCAC GCTGCCTCCTTGAAAGAGAG 55.0 55.0 58.1 58.3 835-866 bp 1.0 82.6
trnT-GGU-trnE-UUC_p4 CCATGGCGTTACTCTACCAC TGTCCTGAACCACTAGACGA 55.0 50.0 58.1 57.7 814-845 bp 1.0 82.6
trnT-GGU-trnE-UUC_p5 TGGCGTTACTCTACCACTGA GCTGCCTCCTTGAAAGAGAG 50.0 55.0 58.1 58.3 832-863 bp 1.0 82.5