Hotspot

trnF-GAA-trnL-UAA

LSC · 15830-16252 · 423 bp

Back to Vigna

Pi 0.0276
MarkerSeek score 68.90
Primer available yes
Label trnF-GAA-trnL-UAA

Feature Metadata

label_name
trnF-GAA-trnL-UAA
feature_type
igs
region
LSC
length_bp
423
variable_sites
17
pi
0.0276
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnF-GAA-trnL-UAA_p1 TTTGAACTGGTGACACGAGG TTCAAGTCCCTCTATCCCCA 50.0 50.0 58.1 57.0 485-502 bp 1.0 77.4
trnF-GAA-trnL-UAA_p2 TTTGAACTGGTGACACGAGG ATCATGAGGGTTCAAGTCCC 50.0 50.0 58.1 56.9 495-512 bp 1.0 76.1
trnF-GAA-trnL-UAA_p3 TTTGAACTGGTGACACGAGG GGGTTCAAGTCCCTCTATCC 50.0 55.0 58.1 56.7 488-505 bp 1.0 74.6
trnF-GAA-trnL-UAA_p4 TTTGAACTGGTGACACGAGG GGTTCAAGTCCCTCTATCCC 50.0 55.0 58.1 56.7 487-504 bp 1.0 74.6
trnF-GAA-trnL-UAA_p5 TTTGAACTGGTGACACGAGG GTTCAAGTCCCTCTATCCCC 50.0 55.0 58.1 56.7 486-503 bp 1.0 74.6