Hotspot

trnH-GUG-psbA

LSC · 3021-3296 · 276 bp

Back to Vigna

Pi 0.0348
MarkerSeek score 72.60
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
276
variable_sites
14
pi
0.0348
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.97
markerseek_score
72.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG CCCTCTAGATCTAGCTGCGA 55.0 55.0 58.1 57.8 313-343 bp 1.0 84.8
trnH-GUG-psbA_p2 GATCCACTTGGCTACATCCG TCCCTCTAGATCTAGCTGCG 55.0 55.0 58.1 57.8 314-344 bp 1.0 84.8
trnH-GUG-psbA_p3 CAATCCACTGCCTTGATCCA CCCTCTAGATCTAGCTGCGA 50.0 55.0 57.9 57.8 327-357 bp 1.0 84.6
trnH-GUG-psbA_p4 CAATCCACTGCCTTGATCCA TCCCTCTAGATCTAGCTGCG 50.0 55.0 57.9 57.8 328-358 bp 1.0 84.6
trnH-GUG-psbA_p5 AATCCACTGCCTTGATCCAC CCCTCTAGATCTAGCTGCGA 50.0 55.0 57.9 57.8 326-356 bp 1.0 84.6