Hotspot

ndhE

SSC · 120094-120399 · 306 bp

Back to Vietsenia

Pi 0.00327

MarkerSeek score 40.60

Primer available yes

Label ndhE

Feature Metadata

label_name: ndhE
feature_type: gene
region: SSC
length_bp: 306
variable_sites: 1
pi: 0.00327
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE_p1	GCCTTACATCCATCCCAAGG	TTTGGACTGTTGGGAATGGG	55.0	50.0	58.0	58.0	1129-1149 bp	1.0	81.0
ndhE_p2	ACCCATGCTTCGAGTTGTTT	TTTGGACTGTTGGGAATGGG	45.0	50.0	58.0	58.0	1256-1276 bp	1.0	80.8
ndhE_p3	CGTGGAGCAGAAGCAATTTG	TTTGGACTGTTGGGAATGGG	50.0	50.0	58.0	58.0	1153-1173 bp	1.0	80.8
ndhE_p4	GCTTCGAGTTGTTTCATGCC	TTTGGACTGTTGGGAATGGG	50.0	50.0	58.0	58.0	1250-1270 bp	1.0	80.7
ndhE_p5	TCGTGGAGCAGAAGCAATTT	TTTGGACTGTTGGGAATGGG	45.0	50.0	58.1	58.0	1154-1174 bp	1.0	80.2