Hotspot

ndhI-ndhA

SSC · 122084-122179 · 96 bp

Back to Vietsenia

Pi 0.0444

MarkerSeek score 67.60

Primer available yes

Label ndhI-ndhA

Feature Metadata

label_name: ndhI-ndhA
feature_type: igs
region: SSC
length_bp: 96
variable_sites: 4
pi: 0.0444
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 67.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI-ndhA_p1	TTTCGCATACCGGTTTCCAA	TACGGTTCTGGACTAGCGAT	45.0	50.0	58.1	58.0	951-957 bp	1.0	80.3
ndhI-ndhA_p2	TTTTCGCATACCGGTTTCCA	TACGGTTCTGGACTAGCGAT	45.0	50.0	58.1	58.0	952-958 bp	1.0	80.3
ndhI-ndhA_p3	TTTCGCATACCGGTTTCCAA	TACCCGAAGCAGAGGAAGAA	45.0	50.0	58.1	58.1	757-763 bp	1.0	80.3
ndhI-ndhA_p4	TCGCATACCGGTTTCCAATT	TACGGTTCTGGACTAGCGAT	45.0	50.0	57.9	58.0	949-955 bp	1.0	80.2
ndhI-ndhA_p5	TTCGCATACCGGTTTCCAAT	TACGGTTCTGGACTAGCGAT	45.0	50.0	57.9	58.0	950-956 bp	1.0	80.2