Hotspot

psaC-ndhE

SSC · 119862-120093 · 232 bp

Back to Vietsenia

Pi 0.0431

MarkerSeek score 74.40

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 232
variable_sites: 10
pi: 0.0431
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	GCCTTACATCCATCCCAAGG	TTATAGCTATTGCCGCAGCC	55.0	50.0	58.0	58.2	449-467 bp	1.0	80.1
psaC-ndhE_p2	CGGACACACTGAGTACATCC	TTATAGCTATTGCCGCAGCC	55.0	50.0	57.7	58.2	398-416 bp	1.0	78.2
psaC-ndhE_p3	GCCTTACATCCATCCCAAGG	GCGGCTATTGGGTTAGCTAT	55.0	50.0	58.0	57.5	426-444 bp	1.0	77.9
psaC-ndhE_p4	GCCTTACATCCATCCCAAGG	AGCGGCTATTGGGTTAGCTA	55.0	50.0	58.0	58.6	427-445 bp	1.0	77.1
psaC-ndhE_p5	CGGACACACTGAGTACATCC	GCGGCTATTGGGTTAGCTAT	55.0	50.0	57.7	57.5	375-393 bp	1.0	76.1