Hotspot

ndhF-rpl32

SSC · 115078-116007 · 930 bp

Back to Vietsenia

Pi 0.0261

MarkerSeek score 64.60

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 930
variable_sites: 23
pi: 0.0261
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TGGGAAGTGGAATGAGAGGT	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.0	58.0	1445-1488 bp	1.0	85.1
ndhF-rpl32_p2	TGGCAACTGCACCTGTAAAT	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.0	58.0	2111-2154 bp	1.0	85.0
ndhF-rpl32_p3	TGGCAACTGCACCTGTAAAT	CAGCGTGTCTACCGATTTCA	45.0	50.0	58.0	58.0	2112-2155 bp	1.0	85.0
ndhF-rpl32_p4	TGGGAAGTGGAATGAGAGGT	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.0	58.0	1446-1489 bp	1.0	85.0
ndhF-rpl32_p5	CGCCTACACGATTCGTTACA	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.1	58.0	1949-1992 bp	1.0	84.3