Hotspot

rpl14-rpl16

LSC · 82940-83086 · 147 bp

Back to Vietsenia

Pi 0.021

MarkerSeek score 67.10

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: LSC
length_bp: 147
variable_sites: 3
pi: 0.021
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	CGCGACATTCAAATGGGTTT	CAATAGCGGCGTCCAAAATG	45.0	50.0	57.7	58.2	229-233 bp	1.0	78.3
rpl14-rpl16_p2	CGCGACATTCAAATGGGTTT	ATTTCAATAGCGGCGTCCAA	45.0	45.0	57.7	58.3	233-237 bp	1.0	77.6
rpl14-rpl16_p3	CGCGACATTCAAATGGGTTT	TCAATAGCGGCGTCCAAAAT	45.0	45.0	57.7	58.3	230-234 bp	1.0	77.6
rpl14-rpl16_p4	CACATCAATTTTCGAGCCCC	CAATAGCGGCGTCCAAAATG	50.0	50.0	57.4	58.2	258-262 bp	1.0	76.2
rpl14-rpl16_p5	CGCGACATTCAAATGGGTTT	TTCAATAGCGGCGTCCAAAA	45.0	45.0	57.7	58.5	231-235 bp	1.0	75.8