Hotspot

psbE-petL

LSC · 65907-67039 · 1133 bp

Back to Vietsenia

Pi 0.008

MarkerSeek score 64.00

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1133
variable_sites: 9
pi: 0.008
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	GCTGAGGTTATAGTTAAAGCTGC	50.0	43.5	57.8	57.6	1219-1241 bp	1.0	52.6
psbE-petL_p2	ACGAATAACCAACCCGCAAT	GCTGAGGTTATAGTTAAAGCTGC	45.0	43.5	58.2	57.6	1305-1327 bp	1.0	52.4
psbE-petL_p3	TGCTGACGAATAACCAACCC	GCTGAGGTTATAGTTAAAGCTGC	50.0	43.5	58.2	57.6	1310-1332 bp	1.0	52.3
psbE-petL_p4	GTTCTCCTGTGCTTCCAGAC	GCTGAGGTTATAGTTAAAGCTGC	55.0	43.5	58.2	57.6	1214-1236 bp	1.0	52.2
psbE-petL_p5	TCTCCTGTGCTTCCAGACAT	GCTGAGGTTATAGTTAAAGCTGC	50.0	43.5	58.4	57.6	1212-1234 bp	1.0	51.0