Hotspot

petA-psbJ

LSC · 64201-65099 · 899 bp

Back to Vietsenia

Pi 0.02

MarkerSeek score 70.30

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 899
variable_sites: 18
pi: 0.02
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ACGTGTCCAAGGTCTTTTGT	ATACTCAGGGTTGGGCTCAT	45.0	50.0	57.6	58.1	1039-1061 bp	1.0	77.3
petA-psbJ_p2	ACGTGTCCAAGGTCTTTTGT	GTTCATACTCAGGGTTGGGC	45.0	55.0	57.6	58.2	1043-1065 bp	1.0	76.1
petA-psbJ_p3	ACGTGTCCAAGGTCTTTTGT	TTCATACTCAGGGTTGGGCT	45.0	50.0	57.6	58.3	1042-1064 bp	1.0	75.4
petA-psbJ_p4	ACGTGTCCAAGGTCTTTTGT	GGTTCATACTCAGGGTTGGG	45.0	55.0	57.6	57.6	1044-1066 bp	1.0	74.7
petA-psbJ_p5	TCCATTACGTGTCCAAGGTC	ATACTCAGGGTTGGGCTCAT	50.0	50.0	57.2	58.1	1045-1067 bp	1.0	74.5