Hotspot

psaI-pafII

LSC · 60948-61393 · 446 bp

Back to Vietsenia

Pi 0.00937

MarkerSeek score 62.10

Primer available yes

Label psaI-pafII

Feature Metadata

label_name: psaI-pafII
feature_type: igs
region: LSC
length_bp: 446
variable_sites: 4
pi: 0.00937
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-pafII_p1	ATTTCCGGCAATTGCAATGG	TATGTTCGGATCGCCAACTC	45.0	50.0	58.0	57.8	510-529 bp	1.0	81.6
psaI-pafII_p2	ATTTCCGGCAATTGCAATGG	AAGGATCAAGGCCCAGAAGA	45.0	50.0	58.0	58.3	572-591 bp	1.0	80.3
psaI-pafII_p3	ATTTCCGGCAATTGCAATGG	AGGATCAAGGCCCAGAAGAA	45.0	50.0	58.0	58.3	571-590 bp	1.0	80.3
psaI-pafII_p4	ATTTCCGGCAATTGCAATGG	TCGAGACCCCACTATACGTT	45.0	50.0	58.0	57.6	539-558 bp	1.0	79.7
psaI-pafII_p5	ATTTCCGGCAATTGCAATGG	TTCGAGACCCCACTATACGT	45.0	50.0	58.0	57.6	540-559 bp	1.0	79.7