Hotspot

rpoB-trnC-GCA

LSC · 27073-28537 · 1465 bp

Back to Vietsenia

Pi 0.0153

MarkerSeek score 68.00

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1465
variable_sites: 20
pi: 0.0153
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	TCACTTCCATCCACCAGCAT	CCGGATTTGAACTGGGGAAA	50.0	50.0	59.0	58.1	1540-1547 bp	1.0	76.3
rpoB-trnC-GCA_p2	TTCCCTCACTTCCATCCACC	CCGGATTTGAACTGGGGAAA	55.0	50.0	59.0	58.1	1545-1552 bp	1.0	76.3
rpoB-trnC-GCA_p3	GACATTCCCTCACTTCCATCC	CCGGATTTGAACTGGGGAAA	52.4	50.0	58.1	58.1	1549-1556 bp	1.0	75.8
rpoB-trnC-GCA_p4	CATTCCCTCACTTCCATCCA	CCGGATTTGAACTGGGGAAA	50.0	50.0	56.9	58.1	1547-1554 bp	1.0	75.5
rpoB-trnC-GCA_p5	ATTCCCTCACTTCCATCCAC	CCGGATTTGAACTGGGGAAA	50.0	50.0	56.9	58.1	1546-1553 bp	1.0	75.5