Hotspot

atpF-atpH

LSC · 13058-13584 · 527 bp

Back to Vietsenia

Pi 0.0282

MarkerSeek score 64.90

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 527
variable_sites: 14
pi: 0.0282
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	GGCCCAAGGAAAGGAAAGAA	CTGTTATTTGCGAATCCTTTTGT	50.0	34.8	58.0	56.3	580-591 bp	1.0	48.5
atpF-atpH_p2	ATTAAACCCGAAACTCCCGG	CTGTTATTTGCGAATCCTTTTGT	50.0	34.8	57.9	56.3	612-623 bp	1.0	47.6
atpF-atpH_p3	GGCCCAAGGAAAGGAAAGAA	TGTTATTTGCGAATCCTTTTGT	50.0	31.8	58.0	55.2	579-590 bp	1.0	47.3
atpF-atpH_p4	ATTAAACCCGAAACTCCCGG	TGTTATTTGCGAATCCTTTTGT	50.0	31.8	57.9	55.2	611-622 bp	1.0	46.3
atpF-atpH_p5	TTGGCCCAAGGAAAGGAAAG	CTGTTATTTGCGAATCCTTTTGT	50.0	34.8	58.3	56.3	582-593 bp	1.0	46.3