Hotspot

trnS-GCU-trnR-UCU

LSC · 7943-9744 · 1802 bp

Back to Vietsenia

Pi 0.0117

MarkerSeek score 65.10

Primer available yes

Label trnS-GCU-trnR-UCU

Feature Metadata

label_name: trnS-GCU-trnR-UCU
feature_type: igs
region: LSC
length_bp: 1802
variable_sites: 21
pi: 0.0117
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnR-UCU_p1	CAATCCGACGCTTTAGTCCA	AGAACTTGCGTCCAATAGGA	50.0	45.0	58.0	56.6	1915-1927 bp	1.0	69.5
trnS-GCU-trnR-UCU_p2	AATCCGACGCTTTAGTCCAC	AGAACTTGCGTCCAATAGGA	50.0	45.0	58.0	56.6	1914-1926 bp	1.0	69.5
trnS-GCU-trnR-UCU_p3	CAATCCGACGCTTTAGTCCA	ACATAGAAGAACTTGCGTCCA	50.0	42.9	58.0	57.3	1922-1934 bp	1.0	67.6
trnS-GCU-trnR-UCU_p4	AATCCGACGCTTTAGTCCAC	ACATAGAAGAACTTGCGTCCA	50.0	42.9	58.0	57.3	1921-1933 bp	1.0	67.6
trnS-GCU-trnR-UCU_p5	CAATCCGACGCTTTAGTCCA	AAGAACTTGCGTCCAATAGGA	50.0	42.9	58.0	57.3	1916-1928 bp	1.0	67.5