Hotspot

rps16-trnQ-UUG

LSC · 5711-6701 · 991 bp

Back to Vietsenia

Pi 0.0192

MarkerSeek score 63.10

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 991
variable_sites: 19
pi: 0.0192
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	CGTTGCTTTCTACCACATCG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.2	58.1	1056-1064 bp	1.0	74.6
rps16-trnQ-UUG_p2	GCACGTTGCTTTCTACCACA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	59.1	58.1	1059-1067 bp	1.0	72.4
rps16-trnQ-UUG_p3	TAAGAATCCACGGACGATCC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	56.8	58.1	1092-1100 bp	1.0	71.5
rps16-trnQ-UUG_p4	CGTTGCTTTCTACCACATCG	TTCGGAGGTTCGAATCCTTC	50.0	50.0	57.2	57.3	1060-1068 bp	1.0	69.9
rps16-trnQ-UUG_p5	CGTTGCTTTCTACCACATCG	TAAGGCAACGGGTTTTGGTC	50.0	50.0	57.2	58.7	1086-1094 bp	1.0	69.7