Hotspot

ycf2

IRa · 146981-153235 · 6255 bp

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Pi 0.218

MarkerSeek score 57.40

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: IRa
length_bp: 6255
variable_sites: 332
pi: 0.218
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.24
markerseek_score: 57.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	GATACGGGCCTTTTCCACAT	TTCTTTTGTCCCTTCGAGGC	50.0	50.0	57.9	58.1	1501 bp	0.5	58.7
ycf2_p2	CTCTCCCCCAAAAAGCATGT	TTCTTTTGTCCCTTCGAGGC	50.0	50.0	58.1	58.1	1607 bp	0.5	58.6
ycf2_p3	GATACGGGCCTTTTCCACAT	CCGGGATGTGATATGGTTCC	50.0	55.0	57.9	57.8	1395 bp	0.5	58.1
ycf2_p4	GATACCCGTGACTCGATTGG	TTCTTTTGTCCCTTCGAGGC	55.0	50.0	57.9	58.1	1638 bp	0.5	58.1
ycf2_p5	CTCTCCCCCAAAAAGCATGT	CCGGGATGTGATATGGTTCC	50.0	55.0	58.1	57.8	1501 bp	0.5	58.0