Hotspot

rpl23

IRa · 153563-153844 · 282 bp

Back to Vicatia

Pi 0.389

MarkerSeek score 63.10

Primer available yes

Label rpl23

Feature Metadata

label_name: rpl23
feature_type: gene
region: IRa
length_bp: 282
variable_sites: 28
pi: 0.389
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.26
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl23_p1	GAATGGTTAAAGCGCCCAAC	ACTGTCTACGGTTCGATTGC	50.0	50.0	58.0	58.0	587 bp	0.5	59.9
rpl23_p2	GAATGGTTAAAGCGCCCAAC	CCACAACGATGCTGTCCATA	50.0	50.0	58.0	58.0	640 bp	0.5	59.7
rpl23_p3	GAATGGTTAAAGCGCCCAAC	TCTGTCGGTGCTTGAAACAA	50.0	45.0	58.0	58.0	1148 bp	0.5	59.7
rpl23_p4	GAATGGTTAAAGCGCCCAAC	TTCTGTCGGTGCTTGAAACA	50.0	45.0	58.0	58.0	1149 bp	0.5	59.7
rpl23_p5	GATTGCTTGTTGAACCCTGC	ACTGTCTACGGTTCGATTGC	50.0	50.0	57.9	58.0	904 bp	0.5	59.4