Hotspot

ccsA-ndhD

SSC · 117167-117383 · 217 bp

Back to Vicatia

Pi 0.102

MarkerSeek score 80.10

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 217
variable_sites: 22
pi: 0.102
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	GGCGTCTATGGGCTTTCTTA	CAGTTGACAAGGTCGAGGTT	50.0	50.0	57.4	57.8	367-372 bp	1.0	78.4
ccsA-ndhD_p2	GGCGTCTATGGGCTTTCTTA	AGTTGACAAGGTCGAGGTTC	50.0	50.0	57.4	57.5	366-371 bp	1.0	76.1
ccsA-ndhD_p3	GGCGTCTATGGGCTTTCTTA	GTTGACAAGGTCGAGGTTCT	50.0	50.0	57.4	57.5	365-370 bp	1.0	76.1
ccsA-ndhD_p4	GGCGTCTATGGGCTTTCTTA	TCAGTTGACAAGGTCGAGGT	50.0	50.0	57.4	58.6	368-373 bp	1.0	75.5
ccsA-ndhD_p5	GGCGTCTATGGGCTTTCTTA	ATCAGTTGACAAGGTCGAGG	50.0	50.0	57.4	57.2	369-374 bp	1.0	74.2