Hotspot

rps4-trnT-UGU

LSC · 47122-47464 · 343 bp

Back to Vicatia

Pi 0.129

MarkerSeek score 83.80

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 343
variable_sites: 43
pi: 0.129
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 83.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	GTTAGTCCCGGTAAAGCTCC	CTCAGAGGTTAGAGCATCGC	55.0	55.0	57.7	57.9	471-472 bp	1.0	85.3
rps4-trnT-UGU_p2	GTTAGTCCCGGTAAAGCTCC	ATGGTCATCGGTTCGATTCC	55.0	50.0	57.7	57.8	439-440 bp	1.0	85.1
rps4-trnT-UGU_p3	AGATCACTTCCGGCTCTAGG	CTCAGAGGTTAGAGCATCGC	55.0	55.0	58.3	57.9	501-502 bp	1.0	84.8
rps4-trnT-UGU_p4	AGATCACTTCCGGCTCTAGG	ATGGTCATCGGTTCGATTCC	55.0	50.0	58.3	57.8	469-470 bp	1.0	84.5
rps4-trnT-UGU_p5	TTTTTGAAACGAGGTCCCCG	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.7	57.9	438-439 bp	1.0	82.9