Hotspot

pafI-trnS-GGA

LSC · 45396-46114 · 719 bp

Back to Vicatia

Pi 0.0547

MarkerSeek score 86.10

Primer available yes

Label pafI-trnS-GGA

Feature Metadata

label_name: pafI-trnS-GGA
feature_type: igs
region: LSC
length_bp: 719
variable_sites: 36
pi: 0.0547
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 86.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
pafI-trnS-GGA_p1	CATTTATCCGCGATCTAGGCA	CCCTCGGTAAACAAAAGCCT	47.6	50.0	58.0	58.1	808-915 bp	1.0	78.3
pafI-trnS-GGA_p2	CATTTATCCGCGATCTAGGCA	ACGGAAAGAGAGGGATTCGA	47.6	50.0	58.0	58.2	829-936 bp	1.0	77.7
pafI-trnS-GGA_p3	CATTTATCCGCGATCTAGGCA	TTCCAATGCTACGCCTTCAA	47.6	45.0	58.0	57.8	779-886 bp	1.0	77.6
pafI-trnS-GGA_p4	CATTTATCCGCGATCTAGGCA	AGTTCCAATGCTACGCCTTC	47.6	50.0	58.0	58.3	781-888 bp	1.0	77.0
pafI-trnS-GGA_p5	CATTTATCCGCGATCTAGGCA	ACCCTCGGTAAACAAAAGCC	47.6	50.0	58.0	58.4	809-916 bp	1.0	75.9