Hotspot

rpl16

LSC · 83979-85446 · 1468 bp

Back to Viburnum

Pi 0.0124

MarkerSeek score 60.20

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1468
variable_sites: 153
pi: 0.0124
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	AAACCTGTGCCTGCGATTTT	GGGTTGAATAAAAATTCGATTGACC	45.0	36.0	59.0	57.5	1561-1581 bp	0.88	39.1
rpl16_p2	AACCTGTGCCTGCGATTTTT	GGGTTGAATAAAAATTCGATTGACC	45.0	36.0	59.0	57.5	1560-1580 bp	0.88	39.1
rpl16_p3	AAACCTGTGCCTGCGATTT	GGGTTGAATAAAAATTCGATTGACC	47.4	36.0	58.3	57.5	1561-1581 bp	0.88	39.1
rpl16_p4	AACCTGTGCCTGCGATTTT	GGGTTGAATAAAAATTCGATTGACC	47.4	36.0	58.3	57.5	1560-1580 bp	0.88	39.1
rpl16_p5	ACCTGTGCCTGCGATTTTT	GGGTTGAATAAAAATTCGATTGACC	47.4	36.0	58.3	57.5	1559-1579 bp	0.88	39.1