Hotspot

ndhF

SSC · 113594-115831 · 2238 bp

Back to Viburnum

Pi 0.00712

MarkerSeek score 44.20

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2238
variable_sites: 99
pi: 0.00712
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GATGGCCTTGTCCACACTAG	GTCTTTTGGAAAGACGCAGA	55.0	45.0	58.0	56.3	2527-2590 bp	0.84	64.2
ndhF_p2	GATGGCCTTGTCCACACTAG	GTCTTTTGGAAAGACGCAGAA	55.0	42.9	58.0	57.0	2527-2590 bp	0.84	62.1
ndhF_p3	AAGAGGGATCCACCGAAGAA	GTCTTTTGGAAAGACGCAGA	50.0	45.0	58.0	56.3	2606-2999 bp	0.28	43.6
ndhF_p4	AGGGATCCACCGAAGAAGAT	GTCTTTTGGAAAGACGCAGA	50.0	45.0	57.8	56.3	2603-2996 bp	0.28	42.5
ndhF_p5	AAGAGGGATCCACCGAAGAA	GTCTTTTGGAAAGACGCAGAA	50.0	42.9	58.0	57.0	2606-2999 bp	0.28	41.6