Hotspot

ndhD-psaC

SSC · 120972-121098 · 127 bp

Back to Viburnum

Pi 0.0689

MarkerSeek score 68.10

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 127
variable_sites: 30
pi: 0.0689
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	CTCCCGCCGTGTACAAAATA	AGAGGACTGTGTCGGTTGTA	50.0	50.0	58.0	58.0	767-777 bp	1.0	88.2
ndhD-psaC_p2	CTCCCGCCGTGTACAAAATA	AAATTGCTTCTGCCCCAAGA	50.0	45.0	58.0	58.0	789-799 bp	1.0	88.0
ndhD-psaC_p3	CTCCCGCCGTGTACAAAATA	AATTGCTTCTGCCCCAAGAA	50.0	45.0	58.0	58.0	788-798 bp	1.0	88.0
ndhD-psaC_p4	TAATCGGCGATTTGACAGCA	AGAGGACTGTGTCGGTTGTA	45.0	50.0	58.0	58.0	929-939 bp	1.0	87.2
ndhD-psaC_p5	TAATCGGCGATTTGACAGCA	AATTGCTTCTGCCCCAAGAA	45.0	45.0	58.0	58.0	950-960 bp	1.0	87.0