Hotspot

ndhF-rpl32

SSC · 115832-117087 · 1256 bp

Back to Viburnum

Pi 0.0243

MarkerSeek score 71.10

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1256
variable_sites: 124
pi: 0.0243
unique_haplotype_count: 23
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GTTGCCGTCGGAAAAAGAAG	TTAACGCTGCCCAATATCCC	50.0	50.0	58.0	58.0	1364-1456 bp	0.96	89.0
ndhF-rpl32_p2	TGCCGTCGGAAAAAGAAGAA	TTAACGCTGCCCAATATCCC	45.0	50.0	58.0	58.0	1362-1454 bp	0.96	88.9
ndhF-rpl32_p3	GTTGCCGTCGGAAAAAGAAG	GTTTGGGTTTGTCGCACAAA	50.0	45.0	58.0	58.0	1434-1526 bp	0.92	87.2
ndhF-rpl32_p4	TGCCGTCGGAAAAAGAAGAA	GTTTGGGTTTGTCGCACAAA	45.0	45.0	58.0	58.0	1432-1524 bp	0.92	87.2
ndhF-rpl32_p5	TTGCCGTCGGAAAAAGAAGA	GTTTGGGTTTGTCGCACAAA	45.0	45.0	58.0	58.0	1433-1525 bp	0.92	87.2