Hotspot

rps8-rpl14

LSC · 83273-83471 · 199 bp

Back to Viburnum

Pi 0.0157

MarkerSeek score 59.50

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 199
variable_sites: 22
pi: 0.0157
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	TCAGCAATAGTATCCCTACCCA	GAACTCGAGTTTTTGGTGCG	45.5	50.0	57.8	58.0	296-315 bp	0.96	68.2
rps8-rpl14_p2	TCAGCAATAGTATCCCTACCCA	ACTCGAGTTTTTGGTGCGAT	45.5	45.0	57.8	58.1	294-313 bp	0.96	67.4
rps8-rpl14_p3	TCAGCAATAGTATCCCTACCCA	AACTCGAGTTTTTGGTGCGA	45.5	45.0	57.8	58.3	295-314 bp	0.96	65.7
rps8-rpl14_p4	AGATGCTATTCGAACCACTCT	GAACTCGAGTTTTTGGTGCG	42.9	50.0	56.5	58.0	357-376 bp	0.96	65.6
rps8-rpl14_p5	GTCAGCAATAGTATCCCTACCC	GAACTCGAGTTTTTGGTGCG	50.0	50.0	57.5	58.0	297-316 bp	0.96	65.6