Hotspot

trnP-UGG-psaJ

LSC · 69427-69815 · 389 bp

Back to Viburnum

Pi 0.0197

MarkerSeek score 59.80

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 389
variable_sites: 60
pi: 0.0197
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	AAAACAAACGCGCTACCAAG	GACCTGCTAAAGACCCGAAC	45.0	55.0	57.9	58.3	491-500 bp	1.0	86.6
trnP-UGG-psaJ_p2	CAAAACAAACGCGCTACCAA	GACCTGCTAAAGACCCGAAC	45.0	55.0	58.2	58.3	492-501 bp	1.0	86.2
trnP-UGG-psaJ_p3	TTGTACCCAAAACAAACGCG	GACCTGCTAAAGACCCGAAC	45.0	55.0	57.8	58.3	499-508 bp	1.0	86.0
trnP-UGG-psaJ_p4	AAAACAAACGCGCTACCAAG	AGACCTGCTAAAGACCCGAA	45.0	50.0	57.9	58.4	492-501 bp	1.0	86.0
trnP-UGG-psaJ_p5	AAAACAAACGCGCTACCAAG	CTGCTAAAGACCCGAACCAT	45.0	50.0	57.9	57.6	488-497 bp	1.0	85.6