Hotspot

petL-petG

LSC · 68674-68848 · 175 bp

Back to Viburnum

Pi 0.0156

MarkerSeek score 60.30

Primer available yes

Label petL-petG

Feature Metadata

label_name: petL-petG
feature_type: igs
region: LSC
length_bp: 175
variable_sites: 26
pi: 0.0156
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petL-petG_p1	TTCGGTTTTCTACTAGCGGC	TGATCACCACGCCTGTATTG	50.0	50.0	58.0	58.0	338-351 bp	0.96	83.5
petL-petG_p2	TTCGGTTTTCTACTAGCGGC	GATCACCACGCCTGTATTGT	50.0	50.0	58.0	58.0	337-350 bp	0.96	83.5
petL-petG_p3	CGGTTTTCTACTAGCGGCTT	TGATCACCACGCCTGTATTG	50.0	50.0	58.0	58.0	336-349 bp	0.96	83.5
petL-petG_p4	CGGTTTTCTACTAGCGGCTT	GATCACCACGCCTGTATTGT	50.0	50.0	58.0	58.0	335-348 bp	0.96	83.5
petL-petG_p5	TTCGGTTTTCTACTAGCGGC	CTGATCACCACGCCTGTATT	50.0	50.0	58.0	57.7	339-352 bp	0.96	81.1