Hotspot

ndhC-trnV-UAC

LSC · 52829-53884 · 1056 bp

Back to Viburnum

Pi 0.016

MarkerSeek score 62.60

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1056
variable_sites: 132
pi: 0.016
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	ATCATATTCGTAAAGAAGAAACAT	AGAAGGTCTACGGTTCGAGT	25.0	50.0	52.6	57.8	1013-1126 bp	1.0	52.0
ndhC-trnV-UAC_p2	ATCATATTCGTAAAGAAGAAACAT	AAAGAGCCCAGTGCACAAAA	25.0	45.0	52.6	58.5	1100-1213 bp	1.0	52.0
ndhC-trnV-UAC_p3	ATCATATTCGTAAAGAAGAAACAT	AAGAGCCCAGTGCACAAAAA	25.0	45.0	52.6	58.5	1099-1212 bp	1.0	52.0
ndhC-trnV-UAC_p4	ATCATATTCGTAAAGAAGAAACAT	GAAGGTCTACGGTTCGAGTC	25.0	55.0	52.6	57.5	1012-1125 bp	1.0	52.0
ndhC-trnV-UAC_p5	ATCATATTCGTAAAGAAGAAACAT	GTTCGAGTCCGTATAGCCCT	25.0	55.0	52.6	58.7	1001-1114 bp	1.0	52.0