Hotspot

petN-psbM

LSC · 29833-31003 · 1171 bp

Back to Viburnum

Pi 0.0107

MarkerSeek score 65.40

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1171
variable_sites: 70
pi: 0.0107
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ACATTTTCCCTTTCGCTCGT	TGCTACTGCACTGTTCATTCT	45.0	42.9	58.1	57.6	596-1305 bp	1.0	76.7
petN-psbM_p2	CCCTTTCGCTCGTAGTATGG	TGCTACTGCACTGTTCATTCT	55.0	42.9	57.9	57.6	589-1298 bp	1.0	76.5
petN-psbM_p3	CCTTTCGCTCGTAGTATGGG	TGCTACTGCACTGTTCATTCT	55.0	42.9	57.9	57.6	588-1297 bp	1.0	76.5
petN-psbM_p4	CTTTCGCTCGTAGTATGGGG	TGCTACTGCACTGTTCATTCT	55.0	42.9	57.9	57.6	587-1296 bp	1.0	76.5
petN-psbM_p5	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.2	57.6	634-1343 bp	1.0	76.2