Hotspot

trnC-GCA-petN

LSC · 29248-29742 · 495 bp

Back to Viburnum

Pi 0.0221

MarkerSeek score 64.90

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 495
variable_sites: 38
pi: 0.0221
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTTGTATCGTTTTGGCGGC	ACGAGCGAAAGGGAAAATGT	45.0	45.0	57.9	58.1	639-1021 bp	1.0	86.4
trnC-GCA-petN_p2	TTTTGTATCGTTTTGGCGGC	CCATACTACGAGCGAAAGGG	45.0	55.0	57.9	57.9	646-1028 bp	1.0	86.1
trnC-GCA-petN_p3	TTTTGTATCGTTTTGGCGGC	CCCATACTACGAGCGAAAGG	45.0	55.0	57.9	57.9	647-1029 bp	1.0	86.1
trnC-GCA-petN_p4	TTTTGTATCGTTTTGGCGGC	CCCCATACTACGAGCGAAAG	45.0	55.0	57.9	57.9	648-1030 bp	1.0	86.1
trnC-GCA-petN_p5	TTTCCCCAGTTCAAATCCGG	ACGAGCGAAAGGGAAAATGT	50.0	45.0	58.1	58.1	583-965 bp	0.96	84.5