Hotspot

atpH-atpI

LSC · 14542-15658 · 1117 bp

Back to Viburnum

Pi 0.00835

MarkerSeek score 64.60

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1117
variable_sites: 53
pi: 0.00835
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AGCCAATCCAGCAGCAATAA	TTTTTGCAACTTTAGCCGCG	45.0	45.0	57.9	58.5	996-1221 bp	0.96	80.5
atpH-atpI_p2	AATAACGGAAGCGGCAGAAA	TTTTTGCAACTTTAGCCGCG	45.0	45.0	58.2	58.5	981-1206 bp	0.96	80.3
atpH-atpI_p3	GCCAATCCAGCAGCAATAAC	TTTTTGCAACTTTAGCCGCG	50.0	45.0	57.8	58.5	995-1220 bp	0.96	80.0
atpH-atpI_p4	ATAACGGAAGCGGCAGAAAT	CCGCGGCTTATATAGGTGAA	45.0	50.0	58.0	57.3	965-1190 bp	0.96	79.9
atpH-atpI_p5	ATAACGGAAGCGGCAGAAAT	TTTTTGCAACTTTAGCCGCG	45.0	45.0	58.0	58.5	980-1205 bp	0.92	79.8