Hotspot

trnK-UUU-rps16

LSC · 4361-5234 · 874 bp

Back to Viburnum

Pi 0.0184

MarkerSeek score 60.90

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 874
variable_sites: 82
pi: 0.0184
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CCCTTTCAGGATCAGTCGTG	AAAAGGGTATGTTGCTGCCA	55.0	45.0	58.0	58.0	1682-1705 bp	1.0	87.3
trnK-UUU-rps16_p2	CCTTTCAGGATCAGTCGTGG	AAAAGGGTATGTTGCTGCCA	55.0	45.0	58.0	58.0	1681-1704 bp	1.0	87.3
trnK-UUU-rps16_p3	TCGACCCAACTTGCAATTGA	AAAAGGGTATGTTGCTGCCA	45.0	45.0	58.0	58.0	1813-1836 bp	1.0	87.0
trnK-UUU-rps16_p4	TCATTCGACCCAACTTGCAA	AAAAGGGTATGTTGCTGCCA	45.0	45.0	58.0	58.0	1817-1833 bp	0.92	82.8
trnK-UUU-rps16_p5	CACACGCATTTCCATTGCAT	AAAAGGGTATGTTGCTGCCA	45.0	45.0	58.0	58.0	2036-2059 bp	0.92	82.3