Hotspot

clpP

LSC · 75470-76113 · 644 bp

Back to Vesalea

Pi 0.0155

MarkerSeek score 54.50

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 644
variable_sites: 15
pi: 0.0155
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	CTGAACGAGTCGCACATACA	TGTTATGGACCTCCCCTGTT	50.0	50.0	58.0	57.9	1316-1331 bp	1.0	81.6
clpP_p2	CTGAACGAGTCGCACATACA	ACGAACATACCCTGTCTCCA	50.0	50.0	58.0	58.1	1575-1590 bp	1.0	81.2
clpP_p3	CTGAACGAGTCGCACATACA	ATGGACAGAAAGCAGTCGAC	50.0	50.0	58.0	57.9	1459-1474 bp	1.0	81.2
clpP_p4	ATGATCTGAACGAGTCGCAC	TGTTATGGACCTCCCCTGTT	50.0	50.0	58.1	57.9	1321-1336 bp	1.0	81.1
clpP_p5	GATCTGAACGAGTCGCACAT	TGTTATGGACCTCCCCTGTT	50.0	50.0	58.1	57.9	1319-1334 bp	1.0	81.1