Hotspot

accD

LSC · 60051-63140 · 3090 bp

Back to Vesalea

Pi 0.0226

MarkerSeek score 65.20

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 3090
variable_sites: 81
pi: 0.0226
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	ACGACGGTTGTCCTATTTGG	CTTAGTCCACACCTGTGCTC	50.0	55.0	57.9	57.9	791-995 bp	1.0	88.9
accD_p2	ACGACGGTTGTCCTATTTGG	ACACCTGTGCTCGAATTTCA	50.0	45.0	57.9	57.7	783-987 bp	1.0	87.6
accD_p3	GAGCACAGGTGTGGACTAAG	ACACCTGTGCTCGAATTTCA	55.0	45.0	57.9	57.7	991-1195 bp	1.0	86.8
accD_p4	TGAAATTCGAGCACAGGTGT	CTTAGTCCACACCTGTGCTC	45.0	55.0	57.7	57.9	1007-1211 bp	1.0	86.7
accD_p5	CGACGGTTGTCCTATTTGGA	CTTAGTCCACACCTGTGCTC	50.0	55.0	57.6	57.9	790-994 bp	1.0	86.6