Hotspot

trnL-CAA-ycf2

IRa · 148859-149578 · 720 bp

Back to Vesalea

Pi 0.00926

MarkerSeek score 66.10

Primer available yes

Label trnL-CAA-ycf2

Feature Metadata

label_name: trnL-CAA-ycf2
feature_type: igs
region: IRa
length_bp: 720
variable_sites: 9
pi: 0.00926
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA-ycf2_p1	AAATGGTAGACACGCGAGAC	GGAACAGGAGAAAGATTTCCCA	50.0	45.5	58.0	58.0	810-823 bp	1.0	65.5
trnL-CAA-ycf2_p2	AAATGGTAGACACGCGAGAC	TGGAACAGGAGAAAGATTTCCC	50.0	45.5	58.0	58.0	811-824 bp	1.0	65.5
trnL-CAA-ycf2_p3	TGAAATGGTAGACACGCGAG	GGAACAGGAGAAAGATTTCCCA	50.0	45.5	58.0	58.0	812-825 bp	1.0	65.5
trnL-CAA-ycf2_p4	TGAAATGGTAGACACGCGAG	TGGAACAGGAGAAAGATTTCCC	50.0	45.5	58.0	58.0	813-826 bp	1.0	65.5
trnL-CAA-ycf2_p5	GCCTTGGTGGTGAAATGGTA	GGAACAGGAGAAAGATTTCCCA	50.0	45.5	58.1	58.0	822-835 bp	1.0	64.9