Hotspot

rpl32-trnL-UAG

SSC · 117630-118422 · 793 bp

Back to Vesalea

Pi 0.0185
MarkerSeek score 57.40
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
793
variable_sites
22
pi
0.0185
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGCGGGCGTATCTCTTTTTA GGAGAGCGGGGACTAATAGT 50.0 55.0 58.1 58.0 1768-1779 bp 0.666667 66.5
rpl32-trnL-UAG_p2 GGCGGGCGTATCTCTTTTTA TGGAGAGCGGGGACTAATAG 50.0 55.0 58.1 58.0 1769-1780 bp 0.666667 66.5
rpl32-trnL-UAG_p3 GGCGGGCGTATCTCTTTTTA AGCGTGTCTACCGATTTCAC 50.0 50.0 58.1 58.0 1233-1244 bp 0.666667 66.5
rpl32-trnL-UAG_p4 GGCGGGCGTATCTCTTTTTA CAGCGTGTCTACCGATTTCA 50.0 50.0 58.1 58.0 1234-1245 bp 0.666667 66.5
rpl32-trnL-UAG_p5 GGCGGGCGTATCTCTTTTTA TCCTAAGAGCAGCGTGTCTA 50.0 50.0 58.1 57.9 1243-1254 bp 0.666667 65.6