Hotspot

trnR-ACG-trnN-GUU

IRb · 113216-113958 · 743 bp

Back to Vesalea

Pi 0.0399

MarkerSeek score 84.40

Primer available yes

Label trnR-ACG-trnN-GUU

Feature Metadata

label_name: trnR-ACG-trnN-GUU
feature_type: igs
region: IRb
length_bp: 743
variable_sites: 44
pi: 0.0399
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 84.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnN-GUU_p1	CCTCGCAATCACTAGCCAAT	AGAAGTTCCGGGAGTTACGA	50.0	50.0	58.0	58.1	1437-1439 bp	1.0	81.8
trnR-ACG-trnN-GUU_p2	TGTCCCCTCCAGTCAAGAAT	AGAAGTTCCGGGAGTTACGA	50.0	50.0	58.0	58.1	1462-1464 bp	1.0	81.7
trnR-ACG-trnN-GUU_p3	CCTTGTTCCTACATGACCCG	AGAAGTTCCGGGAGTTACGA	55.0	50.0	58.0	58.1	1604-1606 bp	1.0	81.6
trnR-ACG-trnN-GUU_p4	CCTTGTTCCTACATGACCCG	TGGGTTGAGAACGGGAATTG	55.0	50.0	58.0	58.1	1558-1560 bp	1.0	81.6
trnR-ACG-trnN-GUU_p5	CCTTGTTCCTACATGACCCG	TTGGTCATGGGTTGAGAACG	55.0	50.0	58.0	58.1	1565-1567 bp	1.0	81.5