Hotspot

ycf2-trnL-CAA

IRb · 98149-98868 · 720 bp

Back to Vesalea

Pi 0.00926

MarkerSeek score 66.10

Primer available yes

Label ycf2-trnL-CAA

Feature Metadata

label_name: ycf2-trnL-CAA
feature_type: igs
region: IRb
length_bp: 720
variable_sites: 9
pi: 0.00926
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2-trnL-CAA_p1	GGAACAGGAGAAAGATTTCCCA	AAATGGTAGACACGCGAGAC	45.5	50.0	58.0	58.0	810-823 bp	1.0	65.5
ycf2-trnL-CAA_p2	TGGAACAGGAGAAAGATTTCCC	AAATGGTAGACACGCGAGAC	45.5	50.0	58.0	58.0	811-824 bp	1.0	65.5
ycf2-trnL-CAA_p3	GGAACAGGAGAAAGATTTCCCA	TGAAATGGTAGACACGCGAG	45.5	50.0	58.0	58.0	812-825 bp	1.0	65.5
ycf2-trnL-CAA_p4	TGGAACAGGAGAAAGATTTCCC	TGAAATGGTAGACACGCGAG	45.5	50.0	58.0	58.0	813-826 bp	1.0	65.5
ycf2-trnL-CAA_p5	GGAACAGGAGAAAGATTTCCCA	GCCTTGGTGGTGAAATGGTA	45.5	50.0	58.0	58.1	822-835 bp	1.0	64.9