Hotspot

psaA-ycf3

LSC · 44052-44864 · 813 bp

Back to Vesalea

Pi 0.0082

MarkerSeek score 67.10

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 813
variable_sites: 10
pi: 0.0082
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	CCAGGTTTAGCCCATTCCTC	ATCAAGCCGCTGAGTATTGG	55.0	50.0	57.9	58.0	985-1006 bp	1.0	81.8
psaA-ycf3_p2	CCAGGTTTAGCCCATTCCTC	GGAAACAGGCTATAGCGCTT	55.0	50.0	57.9	58.0	967-988 bp	1.0	81.8
psaA-ycf3_p3	CCAGGTTTAGCCCATTCCTC	TTGGTTGAAGATCACCAGGC	55.0	50.0	57.9	58.1	918-939 bp	1.0	81.6
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	GGAAACAGGCTATAGCGCTT	50.0	50.0	57.9	58.0	958-979 bp	1.0	81.4
psaA-ycf3_p5	GCCCATTCCTCGAAAGAAGT	ATCAAGCCGCTGAGTATTGG	50.0	50.0	57.9	58.0	976-997 bp	1.0	81.3